Software error:

Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4376
  Illegal SpeI site found at 2622
  Illegal PstI site found at 1105
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4376
  Illegal NheI site found at 3062
  Illegal SpeI site found at 2622
  Illegal PstI site found at 1105
  Illegal NotI site found at 3003
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4376
  Illegal BglII site found at 2518
  Illegal BamHI site found at 2441
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4376
  Illegal SpeI site found at 2622
  Illegal PstI site found at 1105
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4376
  Illegal SpeI site found at 2622
  Illegal PstI site found at 1105
  Illegal AgeI site found at 793
  Illegal AgeI site found at 905
  Illegal AgeI site found at 2083
  Illegal AgeI site found at 2406
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1378
  Illegal BsaI site found at 4382
  Illegal BsaI.rc site found at 1099
  Illegal SapI site found at 1082
  Illegal SapI.rc site found at 6
  

Design Notes

.

Source

synthesized