RNA
Part:BBa_K4662014
Designed by: Julia Wyss Group: iGEM23_UZurich (2023-10-02)
crRNA to block formate acetyltransferase 1
crRNA designed to block the promoter of formate acetyltransferase 1 when tracrRNA is added and expressed with dCas9.
Characterization
Gene Location: NC_000913.3:953670
PAM sequence on antisense strand
Preddicted efficiency: 70%
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Part can be used to manipulate central metabolism leading to more available pyruvate in the cell.
For more information on it's usage BBa_K4662055
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Categories
Parameters
None |