Part:BBa_K4653101:Design
PehA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 209
Illegal NgoMIV site found at 799
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1330
Illegal NgoMIV site found at 1469
Illegal NgoMIV site found at 1519 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 571
Illegal SapI.rc site found at 622
Design Notes
PehA is a polygalacturonic endonuclide derived from Ralstonia solanacearum. It can recognize and cut polygalacturonic acid in pectin of plant cell wall to produce oligogalacturonic acid (OG), which is recognized by pattern recognition receptors on the surface of plant cell membrane and stimulates plant immunity.
Source
Ralstonia solanacearum.
References
[1] Kohorn B D, Kohorn S L. The cell wall-associated kinases, WAKs, as pectin receptors[J]. Frontiers in plant science, 2012, 3: 88.
[2] Ferrari S, Savatin DV, Sicilia F, Gramegna G, Cervone F, Lorenzo GD. Oligogalacturonides: plant damage-associated molecular patterns and regulators of growth and development. Front Plant Sci. 2013 Mar 13;4:49. doi: 10.3389/fpls.2013.00049.
[3] Huang Q, Allen C. Polygalacturonases are required for rapid colonization and full virulence of Ralstonia solanacearum on tomato plants[J]. Physiological and molecular plant pathology, 2000, 57(2): 77-83.
[4] Huang Q, Allen C. Polygalacturonases are required for rapid colonization and full virulence of Ralstonia solanacearum on tomato plants[J]. Physiological and molecular plant pathology, 2000, 57(2): 77-83.
[5] Oligogalacturonides: plant damage-associated molecular patterns and regulators of growth and development