Signalling

Part:BBa_K4634014:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-07)


OmpA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of OmpA is MKKTAIAIAVALAGFATVAQA.Using the GeneOptimizer online tool developed by ThermoFisher, we performed codon optimization and converted the protein sequence of OmpA into a DNA sequence (ATGAAAAAGACCGCAATTGCCATTGCAGTTGCACTGGCAGGTTTTGCAACCGTTGCACAGGCA) which is most suitable for expression in Escherichia coli. OmpA signal peptide should be fused at the N terminal of target protein.



Source

Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.

References

[1] Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.
[2] Pechsrichuang P, Songsiriritthigul C, Haltrich D, Roytrakul S, Namvijtr P, Bonaparte N, Yamabhai M. OmpA signal peptide leads to heterogenous secretion of B. subtilis chitosanase enzyme from E. coli expression system. Springerplus. 2016 Jul 28;5(1):1200. doi: 10.1186/s40064-016-2893-y. PMID: 27516938; PMCID: PMC4963352.