Coding

Part:BBa_K4613001:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-09-29)


Mature Carboxypeptidase A（M-CPA）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for enhanced Escherichia coli expression.


Source

 Bos taurus

References

  1. Xiong L, Peng M, Zhao M, et al. Truncated expression of a carboxypeptidase a from bovine improves its enzymatic properties and detoxification efficiency of ochratoxin A[J]. Toxins, 2020, 12(11): 680.