RNA
gRNA-hIGH3
Part:BBa_K4574035
Designed by: Christopher Pfeiffer Group: iGEM23_Munich (2023-10-06)
RNA guide hIGH-3
CRISPR guide RNA (gRNA), designed to target the human IGH locus, precisely between the J6 joining segment gene and the intronic enhancer Eμ regulatory element. When used with the CRISPR/Cas9 system, this guide sequence, 5'-TCAGGCATCTCGTCCAA^ATG(TGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).
Source
The following basic part was designed to target genetic locus[1].
Organism | Homo sapiens (human) |
Taxonomy | Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo |
Gene | IGH: immunoglobulin heavy locus (NCBI 3492) |
Location | 14q32.33, 105862938→105862960 |
Usage and Biology
This basic part has been used as a component of a CRISPR homology-directed repair system in RAMOS B-cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3492
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Categories
Parameters
None |