Primer

Part:BBa_K4441001:Design

Designed by: Jiachen Lin   Group: iGEM22_Washington   (2022-09-30)


BRAF WT B3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original primer sequence obtained from a journal paper did not work for us so we plug the gene sequence of BRAFV600E into the primer design tools commonly used for LAMP



Source

Generated by Primer Explorer V5 and NEB LAMP Primer Design tool


References