RBS

Part:BBa_K4437500:Design

Designed by: Marian Grell   Group: iGEM22_Calgary   (2022-10-12)


DeadRBS (non-coding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed as a negative control for RBS strength assays, to test the effect of RBS strength on resulting protein expression. As such, the weak translational efficiency of this part was a critical aspect of the part selection process.


Source

This part is sources from a synthetic RBS library compiled by Kosuri et al. (2017).

References