Terminator

Part:BBa_K4398001:Design

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Terminator for Rhodotorula toruloides NP11


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 118
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 118
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 118
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

changed base 332 to a to remove a SapI restrictase recognition site.


Source

https://www.ncbi.nlm.nih.gov/nuccore/NW_015622968.1?report=graph&from=416549&to=416572 from 416133-416572, reversed

References