Composite
Part:BBa_K4385018:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-06)
Lead-inducible surface display
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1809
Illegal NheI site found at 1832
Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1944
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1627
Illegal PstI site found at 462
Illegal PstI site found at 1569 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI site found at 1360
Design Notes
It is mainly composed of pbr promoter (BBa_K4385009) , the anchoring protein Lpp-OmpA(BBa_K4385001, BBa_K4385002), the lead ion adsorbed protein PbrR(BBa_K43885000)and heavy metal detoxification protein( BBa_K4385010).
Source
The basic parts that make up the composite part are all derived from E. coli genomic DNA.