Regulatory

Part:BBa_K4317014:Design

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-12)


PtrnQ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BamHI, EcoRI, and BglII sites are on the front side of this part, and the NdeI site is on the back side.


Source

This part originated from Bacillus subtilis, and we synthesized this DNA fragment from IDT.

References