Coding

Part:BBa_K4298002:Design

Designed by: Buyao Li   Group: iGEM22_Peking   (2022-09-29)


enCas9-PolI5M


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6414
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6414
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4656
    Illegal BamHI site found at 6674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6414
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6414
    Illegal NgoMIV site found at 1566
    Illegal NgoMIV site found at 5567
    Illegal AgeI site found at 5064
    Illegal AgeI site found at 6654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205
    Illegal SapI.rc site found at 1647


Design Notes

Pruduce enCas9-PolI5M with a linker between the two protein and SV40 NLS upstream.

Source

Parts on the plasmid pyEVR.