Composite
Pc-rDAO

Part:BBa_K4225021:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-10)


Pc-rDAO-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1106
    Illegal BamHI site found at 2034
    Illegal BamHI site found at 2288
    Illegal XhoI site found at 904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Spacers are added between the basic parts and it is codon optimized for our chassis DH5α.

Source

All parts are obtained from biobricks

References