Coding

Part:BBa_K422012:Design

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)

Pif3 (AarI C-part)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. Pif3-C is an C-part compatible for C-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

GeneArt. Codon optimized.

References

http://2010.igem.org/Team:ETHZ_Basel

Sato, Hug, Tepperman and Quail: A light-switchable gene promoter system. Nature Biotechnology. 2002; 20.