Plasmid

Part:BBa_K4034008:Design

Designed by: Kleoniki Pylarinou   Group: iGEM21_Athens   (2021-10-17)


pPfuT7, a plasmid for the overexpression of Pfu.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 2855
    Illegal EcoRI site found at 2911
    Illegal EcoRI site found at 3511
    Illegal SpeI site found at 2862
    Illegal PstI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 2855
    Illegal EcoRI site found at 2911
    Illegal EcoRI site found at 3511
    Illegal SpeI site found at 2862
    Illegal PstI site found at 234
    Illegal NotI site found at 2869
    Illegal NotI site found at 2922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 2855
    Illegal EcoRI site found at 2911
    Illegal EcoRI site found at 3511
    Illegal BamHI site found at 290
    Illegal XhoI site found at 284
    Illegal XhoI site found at 2916
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 2855
    Illegal EcoRI site found at 2911
    Illegal EcoRI site found at 3511
    Illegal SpeI site found at 2862
    Illegal PstI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
    Illegal EcoRI site found at 2855
    Illegal EcoRI site found at 2911
    Illegal EcoRI site found at 3511
    Illegal SpeI site found at 2862
    Illegal PstI site found at 234
    Illegal NgoMIV site found at 2610
    Illegal AgeI site found at 432
    Illegal AgeI site found at 556
    Illegal AgeI site found at 1438
    Illegal AgeI site found at 1549
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 319


Design Notes

no


Source

no

References