Part:BBa_K3993002

AsADC

Usage and Biology

This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.

This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.

Figure1. Principle diagram of TAs..

Experimental approach

1. Fragments PCR products Electrophoresis

Figure 2. Gel electrophoresis of amplified fragments..

Lane 1 is target gene AsADC

proof of function

Modeling for predicting the performance of our engineered bacteria to produce tropine

Firstly, we get the polynomial linear regression-2 shown in figure 3. (Data from the published articles, according to references3/4 )

Figure 3..

The R-squared reaches 0.9855, which can be used to predict the performance of our engineered bacteria to produce tropine. Substituting the time and the OD600 value we tested in the laboratory into the model to get figures 4.

Figure 4..

The results show that when the OD600 of our engineered bacteria reaches a certain value, the output of tropine will increase sharply, indicating that our engineered bacteria have great industrial application prospects.

References

4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 689
  Illegal BglII site found at 910
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]