Reporter

Part:BBa_K3962339:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)


J23100 promoter with mCherry for constant expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.


Source

All parts were come from iGEM registry.

References