Composite

Part:BBa_K3939887:Design

Designed by: Haiyang Liu   Group: iGEM21_Tianjin   (2021-09-30)


Cas9 gene regulated by tetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1901
    Illegal BglII site found at 2838
    Illegal BamHI site found at 3656
    Illegal XhoI site found at 5194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 4390
    Illegal AgeI site found at 1259
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The insertion position of tetO in the promoter is critical


Source

from lab

References