Other
EK

Part:BBa_K3934016

Designed by: iGEM Uppsala 2021   Group: iGEM21_Uppsala   (2021-09-30)


Enterokinase cleavage site used to cleave off fusion protein

Entereokinase is a protease which cleaves DNA after the specific sequence Asp-Asp-Asp-Asp-Lys [1]. The sequence can be used in protein expression to cleave off tags or fusion proteins after purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] L. Huang, H. Ruan, W. Gu, Z. Xu, P. Cen, and L. Fan, ‘Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli’, Prep. Biochem. Biotechnol., vol. 37, no. 3, pp. 205–217, 2007, doi: 10.1080/10826060701386695.

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Categories
Parameters
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