Composite

Part:BBa_K3852007

Designed by: YiYang Sun   Group: iGEM21_BIT-China   (2021-10-01)


Pfus+eGFP+CYC1t

The promoter Pfus(activated by Ste12 in the MAPK pathway) was linked to the reporter gene green fluorescent protein gene eGFP and CYC1T to form the detection gene circuit, which was carried into S. cerevisiae cells by shuttle vector pRS42K. Positive colonies were screened for ambenzyl antibody and verified by PCR. Finally, the plasmid was extracted and sequenced to confirm whether the correct plasmid was imported into the colony.


Figure1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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