Composite

Part:BBa_K3805110:Design

Designed by: Yue Tang   Group: iGEM21_BNU-China   (2021-10-01)


mRNA switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 58
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The selection of connexin ensures that the target product can be purified conveniently and efficiently when the fusion protein product is purified


Source

mRNA binding site, yeGFP

References