Coding
DbpA

Part:BBa_K3790001:Design

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-01)


DbpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

5-KpnI-8K (1-50)
5-gca GGTACC atgcagaaaTtgcagaacaccgaaaaaattctgattattctgagccatgg-3

8K-1 (29-87)
5-ttctgattattctgagccatggctgcgcgaccctggaacgcctggaagaaaaaaccggc-3

8K-bridge1 (73-100)
5-CTTCGCGCGGAATGCCGGTTTTTTCTTC-3

8K-2 (88-146)
5-attccgcgcgaagaactgctggtgtatctgacccgcctgtataaccgcggcctgattta-3

8K-bridge2 (133-161)
5-TTCTGCCATTTGCGATAAATCAGGCCGCG-3

8K-3 (147-205)
5-tcgcaaatggcagaaatatggcggcaaaaaatttcgcgaatattgcctgaaaaaccgcg-3

8K-XhoI-3 (184-222)
5-atcCTCGAGttagcgcagaatttcatcgcggtttttcaggcaatattc-3

Source

References