RBS

Part:BBa_K3745005

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-29)


EC6015/Bujard RBS for biosensor

This part is the modified RBS for our biosensor. Ailurus Biotechnology helped us to fine-tune its strength to ensure the rhamnose yield is within the dynamic range of the rhamnose-inducible genetic circuit.

Characterization

The basic Characterization information of this part is listed below:

• Throughput: 2500

• Organism: E. coli

• Strain type: BL21(DE3)

• Temperature: 30°C

• Media: 250 ml LB + 0.1g/L rhamnose (w./v.)

• Period of time: 12 hours

• Plasmid backbone: pSB1C3

Under this condition, the expression of antibiotics resistance gene significantly changes with the concentration of rhamnose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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