Generator

Part:BBa_K3740057

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-09-28)


pR-RBS300-SE-B1006

Description

We replaced the nuclease domain of pyocin S2 with that of colicin E3 to produce a chimeric bacteriocin termed SE.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2497
    Illegal BglII site found at 2202
    Illegal BamHI site found at 1774
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2497
    Illegal NgoMIV site found at 1573
    Illegal AgeI site found at 1663
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None