Composite
pT7-HNMT

Part:BBa_K3647666:Design

Designed by: Natalia Savinkova, Ida Berghten, Astrid Welin, Thalia Rodriquez, Osamudiamen Iyere, Frida Haugskott, Oliver Hild Walett   Group: iGEM20_Linkoping   (2020-08-20)


pT7-Histamine N-methyltransferase (HNMT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 463
    Illegal BsaI.rc site found at 847


Design Notes

Added 6XHIS tag and TEV site in the N-terminus. DNA sequence of HNMT is codon optimized for Escherichia coli. T7 promotor system with 5'UTR added upstream of the HNMT gene. Downstream is a double terminator.


Source

Homo sapiens (HNMT), T7 bacteriophage (transcription and translation), and Tobacco Etch virus (TEV-site).

References