Composite

Part:BBa_K3583009:Design

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Physcomitrella patens PPK with vacuolar import peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3099
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2113
    Illegal XbaI site found at 2191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3099
    Illegal NheI site found at 121
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3099
    Illegal BglII site found at 2839
    Illegal BamHI site found at 353
    Illegal BamHI site found at 1073
    Illegal XhoI site found at 348
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3099
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2113
    Illegal XbaI site found at 2191
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3099
    Illegal XbaI site found at 75
    Illegal XbaI site found at 146
    Illegal XbaI site found at 2113
    Illegal XbaI site found at 2191
    Illegal NgoMIV site found at 770
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3346
    Illegal SapI.rc site found at 1178
    Illegal SapI.rc site found at 1724
    Illegal SapI.rc site found at 1933
    Illegal SapI site found at 3278


Design Notes

The stop codon of the enzyme has to be moved behind the PSI.


Source

Check the pages of each Biobrick.

References