Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.


Status: 500 Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 610
    Illegal NheI site found at 1392
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 610
    Illegal BglII site found at 950
    Illegal BglII site found at 2099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 610
    Illegal PstI site found at 175
    Illegal PstI site found at 1741
    Illegal NgoMIV site found at 528
    Illegal NgoMIV site found at 1119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1401
    Illegal BsaI.rc site found at 1889
    Illegal BsaI.rc site found at 2253


Design Notes

This sequence contain signal peptide for fission yeast. You can't use this sequence directly for other organism like budding yeast.


Source

Signal petpdie is from Cell wall integrity and stress response component 1 of Fission yeast. Protein is from Normal human FGFR sequence withour signal peptide

References