Plasmid_Backbone

Part:BBa_K3482021:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-21)


pAND-MSC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 2007
    Illegal PstI site found at 2047
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1859
    Illegal NheI site found at 19
    Illegal PstI site found at 2007
    Illegal PstI site found at 2047
    Illegal NotI site found at 2318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1859
    Illegal BamHI site found at 4287
    Illegal XhoI site found at 1698
    Illegal XhoI site found at 4958
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 2007
    Illegal PstI site found at 2047
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1859
    Illegal PstI site found at 2007
    Illegal PstI site found at 2047
    Illegal NgoMIV site found at 5074
    Illegal AgeI site found at 105
    Illegal AgeI site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1833
    Illegal BsaI.rc site found at 5189


Design Notes

Temporary


Source

Temporary

References