Part:BBa_K3466007:Design

T7 -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 277
  Illegal AgeI site found at 379
  Illegal AgeI site found at 1152
  Illegal AgeI site found at 1264
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Used a T7 promoter to test for better compatibility with the Sigma 70 Master Mix from Arbor Biosciences.

Source

cgccatgctgactgattgatcgtagtcgatgaattcgcggccgctgTAATACGACTCACTATAGgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggctgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaatcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagtgcgttagcgtacattacagtcagctagct