Part:BBa_K3308093

pKLD66 (pTEV6- maltose binding protein) Backbone

pKLD66

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1210
  Illegal XbaI site found at 6570
  Illegal PstI site found at 1222
  Illegal PstI site found at 2547
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal PstI site found at 1222
  Illegal PstI site found at 2547
  Illegal NotI site found at 1236
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 393
  Illegal BglII site found at 6504
  Illegal XhoI site found at 1143
  Illegal XhoI site found at 1245
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1210
  Illegal XbaI site found at 6570
  Illegal PstI site found at 1222
  Illegal PstI site found at 2547
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1210
  Illegal XbaI site found at 6570
  Illegal PstI site found at 1222
  Illegal PstI site found at 2547
  Illegal NgoMIV site found at 1544
  Illegal NgoMIV site found at 4724
  Illegal NgoMIV site found at 4884
  Illegal NgoMIV site found at 6472
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 91
  Illegal BsaI site found at 2721
  Illegal SapI.rc site found at 3803