Composite
C1

Part:BBa_K3308083:Design

Designed by: Jemy Varghese, Harrison Green, Ripal Sheth, Victor So, Mel Marciesky   Group: iGEM19_Pittsburgh   (2019-10-06)


[NrdJ-1 C (1-4)]-[Tvo VMA C48 N]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1222
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106


Design Notes

Design of this part was based on predicted best site to splice. The splice site was chosen according to biochemical requirements for interactions between either ends of intein.


Source

The source of the intein sequence and characterization data is InBase

References