Device

Part:BBa_K3304000:Design

Designed by: Platon Megagiannis   Group: iGEM19_Thessaloniki   (2019-10-14)


Input Gate with NF-κB p65 consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted PvuII recognition domains to the two ends of the gate by Golden Gate assembly. The insertion of the sequence to Psb1C3 was performed through the Golden Gate assembly by our modified AmilCP construct (part: ). The scar produced through the assembly produces both PvuII sites necessary for our gate to function.


Source

The part is synthetically produced and designed to be inserted into plasmids containing the BioBrick prefix and suffix

References