Generator

Part:BBa_K325209:Design

Designed by: Anja Hohmann and Will Handley   Group: iGEM10_Cambridge   (2010-09-17)


Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334


Design Notes

Removal of existing lux promoter, and replacement with pBAD


Source

From pHK555 provided by James Slock ( http://departments.kings.edu/biology/lux/bacterial.html )

References