Part:BBa_K3190200
GPA1:G Protein Alpha subunit
Gpa1p encodes a guanine nucleotide binding protein that functions as a subunit of the heterotrimeric G protein involved in the pheromone response pathway. Haploid yeast cells exist as one of two mating types, MATa or MATalpha. Mating of haploid cells to form diploids is initiated when the peptide pheromones produced by either cell type (a factor or alpha factor) bind to seven-transmembrane receptor proteins on cells of the opposite type: Ste2p (MATa cells) or Ste3p (MATalpha cells). Pheromone binding leads to activation of the heterotrimeric G-protein composed of alpha subunit Gpa1p, beta subunit Ste4p, and gamma subunit Ste18p. Activation is mediated by exchange of GDP for GTP on the alpha subunit, causing the heterotrimer to dissociate. The resulting Ste4p-Ste18p dimer mediates signal transduction through binding to both the scaffolding protein Ste5p and the PAK kinase Ste20p, causing activation of a MAP kinase cascade (Ste11p, Ste7p, and Fus3p).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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