Part:BBa_K3166004:Experience

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Applications of BBa_K3166004

It was reported that the dehydrosqualene desaturase CrtN from Staphylococcus aureus converts squalene into yellow carotenoid pigments, permitting the colorimetric detection of squalene produced in the cell. Sub-cloning CrtN into the plasmid helps us to screen out squalene synthase with high activity rapidly.

It was sub-cloned into genes below:

pETDuet-1T7-KSS-2T7-CrtN(pET-KN) BBa_K3166997

pETDuet-1T7-NSS-2T7-CrtN(pET-NN) BBa_K3166800

pETDuet-1T7-YSS-2T7-CrtN(pET-YN) BBa_K3166998

pETDuet-1T7-thSQS-2T7-CrtN(pET-thN) BBa_K3166998

pETDuet-rbs-idi-rbs-ispA-rbs-YSS-2T7-CrtN（best composite part BBa_K3166808https://parts.igem.org/Part:BBa_K3166058）

User Reviews

UNIQ59a1df2b652bc124-partinfo-00000000-QINU UNIQ59a1df2b652bc124-partinfo-00000001-QINU