Plasmid_Backbone

Part:BBa_K3163004

Designed by: Burhanuddin Sabuwala, Neha Swaminathan, Shashank H S   Group: iGEM19_IIT-Madras   (2019-10-16)


pCAMBIA 1301 plasmid

pCAMBIA 1301 is a vector used for genetic engineering of plants.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 11806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 11806
    Illegal NheI site found at 2794
    Illegal NheI site found at 6239
    Illegal NotI site found at 4113
    Illegal NotI site found at 5646
    Illegal NotI site found at 6936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 11806
    Illegal BglII site found at 776
    Illegal XhoI site found at 9682
    Illegal XhoI site found at 10776
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 11806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 11806
    Illegal NgoMIV site found at 3558
    Illegal NgoMIV site found at 3732
    Illegal NgoMIV site found at 3782
    Illegal NgoMIV site found at 3786
    Illegal NgoMIV site found at 3813
    Illegal NgoMIV site found at 4312
    Illegal NgoMIV site found at 4670
    Illegal AgeI site found at 6733
    Illegal AgeI site found at 10403
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6636
    Illegal SapI.rc site found at 6864
    Illegal SapI site found at 7327


[edit]
Categories
Parameters
None