Translational_Unit
LytC-Flex-

Part:BBa_K316043:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-27)

Pveg-spoVG-LytC-Flexible Linker-TEV Cleavage Site-AIP-His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1087
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

LytC cell wall binding domain BBa_K316030 was extracted from ‘’Bacillus subtilis’’ genome using PCR with Pfu DNA polymerase. The linker, TEV protease cleavage site and his-tagged auto inducing peptide were produced by direct synthesis by mwg Eurofins[http://www.eurofinsdna.com/home.html]. The two parts were combined using AccI[http://www.neb.com/nebecomm/products/productR0161.asp] restriction endonuclease.