Coding

Part:BBa_K3113012:Design

Designed by: Theresa Keil   Group: iGEM19_Munich   (2019-09-04)


XPA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon Optimisation H62Q for optimal function in mammalian cells. A silent mutation A675T was performed to remove an illegal restriction site.

Source

This fluorescence protein is based on a completely synthetic sequence which was given to the team by PhD Student Dong-Jiunn Jeffery Truong.

References