Part:BBa_K3054014

pcbA5 protein from Dehalococcoides mccartyi CG5 + FLAG-tag

pcbA5 is a polychlorinated biphenyl reductive dehalogenase. Codon optimized for yeast (BBa_K3054005), with Flag Tag

Usage and Biology

PCB dechlorinases: pcbA5

PCB dechlorinases belong to a family of proteins called reductive dehalogenases, that remove chlorine groups from PCB. Removal of PCB from core biphenyl is approached differently by different group of organisms. For eukaryotes, fungi use laccases to dechlorinate PCB, while in bacteria, a modified version of biphenyl degradation pathway helps cells to survive in PCB-rich environments. For the archebacteria Dehalococcoides, it uses this reductive dehalogenase to dechlorinate PCB. This protein was supposed to dechlorinate most of the PCB molecules to lowly chlorinated PCB molecules in our system.

For our experimental purposes, we were trying to target the specific PCB mixture called Aroclor 1260, which was also used by the authors of the paper.

Reference: Donna L. Bedard, PCB dechlorinases revealed at last, PNAS August 19, 2014 111 (33) 11919-11920; first published August 6, 2014 https://doi.org/10.1073/pnas.1412286111

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1056
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 64
  
• 1000
  COMPATIBLE WITH RFC[1000]