Tag

Part:BBa_K3033012

Designed by: Philip Naderev P.Lagniton   Group: iGEM19_UM_Macau   (2019-10-12)


NSP4

Derived from E.coli and synthesized through IDT.

A transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted out of the cell. It codes for a secretion signal sequence which would help our tyrosinase enzyme to be transported into the medium.

Usage and Biology

Team can use this signal sequence in order to secrete their protein into the supernatant

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


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