Part:BBa_K300028:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQa8a32d63aa9064d1-partinfo-00000000-QINU

••••• UNIPV-Pavia iGEM 2010

The parameters that summarize this part quantitative behaviour are reported in the table below. For details about the meaning of these parameters and the estimation methods, see BBa_K300009 Experience page. Sender on low copy plasmid pSB4C5 and Receiver on high copy plasmid pSB1A3 Self-inducible device working not working LB M9 O.D.start K_HSL [nmol/min] Doubling time [min] Scell ratio O.D.start K_HSL [nmol/min] Doubling time [min] Scell ratio BBa_K300028 (wiki name: I15) in pSB4C5 plasmid LC HC 0.32 ± 0.04 ** 8.31 10^-17 ± 3.92 10^-17 ** 33.24 ± 1.27 0.61 ± 0.04 ** 0.15 ± 0.007 1.72 10^-16 ± 6.65 10^-18 65.57 ± 5.99 0.26 ± 0.03

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Absorbance, fluorescence and GFP synthesis rate signals of a typical experiment on this device, obtained as described in BBa_K300009 Experience page.

Growth curve of BBa_K300028 (O.D.600)	Fluorescence curve of BBa_K300028 (G.F.P.)
Fluorescence VS Optical density curve of BBa_K300028	Scell=(dGFP/dt)/O.D.600 of BBa_K300028 and threshold

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