Composite

Part:BBa_K2957023

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, mKate2

Contents: hEF1a, mKate2, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and mKate2 reporter allowing for identifiable expression.

What does it do?: It's responsible for the constitutive expression of the reporter, mKate2. It was important for our project in particular because it was able to show successful transfection in HEK cells

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (https://2019.igem.org/Team:MIT/Parts)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 964
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 964
    Illegal AgeI site found at 81
    Illegal AgeI site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None