Coding

Part:BBa_K2871001:Design

Designed by: Nuttawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-09-24)


EspD Translocon protein. Membrane protein for T3SS docking.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 422
    Illegal XhoI site found at 1045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

a PstI restriction site resides inside the coding sequence. therefore a silent mutation of the relevant codon was made in order to avoid having PstI inside the coding sequence.


Source

obtained by amplification with primers from E. coli. Genomic sequence derived from the paper chaterjee, et al. 2015.

References