Part:BBa_K284022:Design
T4 endolysin under the control of T7 promotor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 322
Illegal AgeI site found at 392 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For more accurate tests about the endolysin effectiveness, this part has to be under the control of an inducible promoter, like the T7 promoter.
Source
This device is a junction of previously designed parts: BBa_I746911 - Designed by Stefan Milde Group: iGEM07_Cambridge (2008-09-30) BBa_K112806 - Designed by Jin Huh Group: iGEM08_UC_Berkeley (2008-10-31) Bba_B0014 - Designed by Reshma Shetty Group: Registry (2003-07-16)
References
Callewaert, L.; Masschalck, B.; Deckers, D.; Nakimbugwe, D.; Atanassova, M.; Aertsen, A.; Michiels, C.W. 2005. Purification of Ivy, a lysozyme inhibitor from Escherichia coli, and characterization of its specificity for various lysozymes. Enzyme Microb. Technol. v. 37, 205-211.
Nakimbugwe, D.; Masschalck, B.; Deckers, D.; Callewaert, L.; Aertsen, A.; Michiels, C.W. 2006. Cell wall substrate specificity of six different lysozymes and lysozyme ihibitory activity of bacterial extracts. FEMS Microbiol. Lett. v. 259, 41-46.
Nakimbugwe, D.; Masschalck, B.; Atanassova, M.; Zewdie-Bosuener, A.; Michiels, C.W. 2006. Comparison of bactericidal activity of six lysozymes at atmospheric pressure and under high hydrostatic pressure. Int. J. Food Microbiol. v. 108, 355-363.