Composite

Part:BBa_K2836002:Design

Designed by: Chang Wei-Tse   Group: iGEM18_SHSID_China   (2018-10-07)


luxCDABEG-luxG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 4171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3169
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5558
    Illegal BsaI.rc site found at 2567
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5883


Design Notes

Part BBa_K325909 is provided within the 2018 Distribution Kit, and Part BBa_K2836001 is synthesized according to the luxG sequence provided by NCBI (Genebank https://www.ncbi.nlm.nih.gov/gene/3280675) without any mutations or codon optimizations. These two parts are then cut and ligated to each other to form the composite part BBa_K2836002.


Source

This is a composite part made up of Part BBa_K325909 and a luxG gene that the 2018 SHSID_China iGEM team synthesized (Part BBa_K2836001).

References