Measurement

Part:BBa_K2786004:Design

Designed by: Takumi Kasahara   Group: iGEM18_Botchan_Lab_Tokyo   (2018-10-03)


nifB promoter->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 828


Design Notes

We selected EcoR1 as preffix and SPe1 as suffix. confining that the nifB promoter did have the -10/-35.

Source

Part K2786002 came from upstream of Paenibacillus polymyxa ATCC15970 nif genes.(nifBHKENXHesA)

References