Coding
Part:BBa_K2770009
Designed by: Jana Anton, Levin Hafa, Leon Schroeder, Sergei Senger Group: iGEM18_TU_Darmstadt (2018-09-30)
Alcohol dehydrogenase (adh)
The alcohol dehydrogenase from Lactobacillus kefir is a NADP+-dependent enzyme, which catalyzes the production of aliphatic and aromatic ketones as well as β-keto esters from their respective alcohol. Here we use the ADH to oxidize cyclohexanol to cyclohexanone and as a recycling agent for the cofactor NADP+, which is needed for the CHMO to function. The ADH coding sequence (CDS) is cloned into the pSB1C3 vector, containing the BioBrick prefix and suffix, a T7/lac Promotor (K921001), an RBS (B0034), an N-terminal 10x His-tag for purification and a GS-Linker. The coding sequence consists of 756 bp which are translated to 252 amino acids. ADH has a molecular mass of 26.8 kDa.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 268
Illegal AgeI site found at 34 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |