Coding

Part:BBa_K2770005

Designed by: Jonathan Achter, Jamina Gerhardus, Christian Hake, Yannik Kaeseberg, Victor Macarron Palacios, Theresa Woermann   Group: iGEM18_TU_Darmstadt   (2018-09-30)


ICL1 - Isocitrate Lyase


ICL1 encodes an isocitrate lyase which catalyzes the key reaction in the glycolic acid cycle. The enzyme transforms isocitrate into glyoxylate and succinate.

For more information about ICL1 have a look on our composit part Baa_K2770007 or our [http://2018.igem.org/Team:TU_Darmstadt/Project/Glycolic_acid/S_cerevisiae wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1627
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None