Coding
Part:BBa_K2770001
Designed by: Luca Brenker, Peter Gockel, Maria Musillo, Elena Nickels, Jan Benedict Spannenkrebs Group: iGEM18_TU_Darmstadt (2018-09-29)
aceA - Isocitrate Lyase
We present the codon optimized gene aceA. As an isocitrate lyase, it converts isocitrate to produce succinate and glyoxylate. For its expression, iGEM TU Darmstadt inserted a T7 lac promotor (BBa_K921000) and a B0034-based ribosomal binding site (BBa_K2380024) upstream of the coding sequence via the BioBrick assembly.
For further characterizations please visit the composite part site or our [http://2018.igem.org/Team:TU_Darmstadt/Project/Glycolic_acid/E_coli wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 106
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |