Coding

Part:BBa_K2770001

Designed by: Luca Brenker, Peter Gockel, Maria Musillo, Elena Nickels, Jan Benedict Spannenkrebs   Group: iGEM18_TU_Darmstadt   (2018-09-29)


aceA - Isocitrate Lyase

We present the codon optimized gene aceA. As an isocitrate lyase, it converts isocitrate to produce succinate and glyoxylate. For its expression, iGEM TU Darmstadt inserted a T7 lac promotor (BBa_K921000) and a B0034-based ribosomal binding site (BBa_K2380024) upstream of the coding sequence via the BioBrick assembly.

Text“ width=

For further characterizations please visit the composite part site or our [http://2018.igem.org/Team:TU_Darmstadt/Project/Glycolic_acid/E_coli wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 106
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None