Composite
Part:BBa_K2686005:Experience
Designed by: Thomas Jordan Group: iGEM18_EPFL (2018-10-07)
The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. Since this part is an intermediary only built for cloning purposes, the part was never expressed by itself in cell-free, but was ligated with inserts (OT1 coding sequences in our case) using Golden Gate assemblies with BsaI.
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UNIQ8ca37bfe8a6ece2f-partinfo-00000001-QINU UNIQ8ca37bfe8a6ece2f-partinfo-00000002-QINU